Recognition of nucleoplasmin by its nuclear transport receptor importin α/β: insights into a complete import complex.

Falces J, Arregi I, Konarev PV, Urbaneja MA, Svergun DI, Taneva SG, Bañuelos S, Biochemistry 49(45):9756-69 (2010) Europe PMC

SASDAD5 – Nucleoplasmin_ImpA_ImpB

Nucleoplasmin_importinA_importinB
MWexperimental 860 kDa
MWexpected 900 kDa
VPorod 1800 nm3
log I(s) 4.07×103 4.07×102 4.07×101 4.07×100
Nucleoplasmin_importinA_importinB small angle scattering data  s, nm-1
ln I(s)
Nucleoplasmin_importinA_importinB Guinier plot ln 4.07×103 Rg: 8.6 nm 0 (8.6 nm)-2 s2
(sRg)2I(s)/I(0)
Nucleoplasmin_importinA_importinB Kratky plot 1.104 0 √3 sRg
p(r)
Nucleoplasmin_importinA_importinB pair distance distribution function Rg: 8.6 nm 0 Dmax: 28 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Nucleoplasmin_importinA_importinB DAMMIF model
log I(s)
 s, nm-1
Nucleoplasmin_importinA_importinB SASREF model
Synchrotron SAXS data from solutions of Nucleoplasmin_ImpA_ImpB in 50 mM Tris-HCL 150 mM NaCl 1.0 mM DTT, pH 7.5 were collected on the EMBL X33 beam line at the DORIS III, DESY storage ring (Hamburg, Germany) using a Pilatus 2M detector (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 1 and 4 mg/ml were measured at 10°C. Two successive 120 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Wavelength = UNKNOWN. Sample detector distance = UNKNOWN

Tags: X33
Nucleoplasmin_importinA_importinB (NP_ImpA_ImpB)
Mol. type   Protein
Organism   Escherichia coli
Olig. state   Other
Mon. MW   900 kDa
Sequence   FASTA