| MWI(0) | 84 | kDa |
| MWexpected | 93 | kDa |
| VPorod | 135 | nm3 |
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log I(s)
8.69×101
8.69×100
8.69×10-1
8.69×10-2
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s, nm-1
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Conformational analysis of a genetically encoded FRET biosensor by SAXS.
Mertens HD, Piljić A, Schultz C, Svergun DI, Biophys J 102(12):2866-75 (2012) Europe PMCSASDAE5 – CYNEX4
CYNEX4 FRET probe, (eYFP-AnnexinA4-eCFP)|
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Fits and models
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| Synchrotron SAXS data from solutions of CYNEX4 in 50 mM HEPES 150 mM NaCl 1 mM EGTA, pH 7.5 were collected on the EMBL X33 beam line at the DORIS III, DESY storage ring (Hamburg, Germany) using a Pilatus 1M-W detector at a sample-detector distance of 2.7 m and at a wavelength of λ = 0.15 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 0.8 and 12.8 mg/ml were measured at 10°C. Eight successive 15 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve. |
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