Quaternary structure of human, Drosophila melanogaster and Caenorhabditis elegans MFE-2 in solution from synchrotron small-angle X-ray scattering.

Mehtälä ML, Haataja TJ, Blanchet CE, Hiltunen JK, Svergun DI, Glumoff T, FEBS Lett 587(4):305-10 (2013) Europe PMC

SASDAG4 – HsMfe2

Peroxisomal multifunctional enzyme type 2
MWI(0) 100 kDa
MWexpected 159 kDa
log I(s) 1.21×102 1.21×101 1.21×100 1.21×10-1
Peroxisomal multifunctional enzyme type 2 small angle scattering data  s, nm-1
ln I(s)
Peroxisomal multifunctional enzyme type 2 Guinier plot ln 1.21×102 Rg: 4.6 nm 0 (4.6 nm)-2 s2
(sRg)2I(s)/I(0)
Peroxisomal multifunctional enzyme type 2 Kratky plot 1.104 0 3 sRg
Dmax: 15 nm

Data validation


There are no models related to this curve.

Synchrotron SAXS data from solutions of HsMfe2 in 20 mM Sodium Phosphate 200 mM NaCl 5% (v/v) Glycerol 1mM Na2EDTA 1 mM NaN3, pH 7.5 were collected on the EMBL X33 beam line at the DORIS III, DESY storage ring (Hamburg, Germany) using a Pilatus 1M-W detector at a sample-detector distance of 2.7 m and at a wavelength of λ = 0.15 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 1.3 and 5.5 mg/ml were measured at 15°C. Eight successive 15 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.

Tags: X33
Peroxisomal multifunctional enzyme type 2 (HsMfe2)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Dimer
Mon. MW   79.7 kDa
 
UniProt   P51659
Sequence   FASTA