| MWexperimental | 44 | kDa |
| MWexpected | 46 | kDa |
| VPorod | 57 | nm3 |
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log I(s)
8.23×103
8.23×102
8.23×101
8.23×100
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s, nm-1
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Molecular mechanism for the action of the anti-CD44 monoclonal antibody MEM-85.
Škerlová J, Král V, Kachala M, Fábry M, Bumba L, Svergun DI, Tošner Z, Veverka V, Řezáčová P, J Struct Biol 191(2):214-23 (2015) Europe PMCSASDAG7 – CD44 HABD scFv MEM-85 complex
Hyaluronate binding domain of CD44 antigenSingle-chain Variable Fragment of Antibody MEM-85
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Fits and models
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Synchrotron SAXS
data from solutions of
CD44 HABD scFv MEM-85 complex
in
PBS, pH 7.4
were collected
on the
EMBL P12 beam line
at the PETRA III storage ring
(DESY; Hamburg, Germany)
using a Pilatus 2M detector
at a sample-detector distance of 3.1 m and
at a wavelength of λ = 0.12 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
Solute concentrations ranging between 1.3 and 5 mg/ml were measured
at 10°C.
20 successive
0.050 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
The low angle data collected at lower concentrations were extrapolated to infinite dilution and merged with the higher concentration data to yield the final composite scattering curve.
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