Structure of a Native-like Aureochrome 1a LOV Domain Dimer from Phaeodactylum tricornutum.

Banerjee A, Herman E, Kottke T, Essen LO, Structure 24(1):171-178 (2016) Europe PMC

SASDAJ8 – Light state solution structure of Aureochrome1a- A´α-LOV-Jα

Aureochrome1a-A´α-LOV-Jα
MWexperimental 63 kDa
MWexpected 36 kDa
VPorod 88 nm3
log I(s) 6.27×101 6.27×100 6.27×10-1 6.27×10-2
Aureochrome1a-A´α-LOV-Jα small angle scattering data  s, nm-1
ln I(s)
Aureochrome1a-A´α-LOV-Jα Guinier plot ln 6.28×101 Rg: 3.3 nm 0 (3.3 nm)-2 s2
(sRg)2I(s)/I(0)
Aureochrome1a-A´α-LOV-Jα Kratky plot 1.104 0 √3 sRg
p(r)
Aureochrome1a-A´α-LOV-Jα pair distance distribution function Rg: 3.5 nm 0 Dmax: 13 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Aureochrome1a-A´α-LOV-Jα DAMMIF model
Synchrotron SAXS data from solutions of Light state solution structure of Aureochrome1a- A´α-LOV-Jα in 10 mM Tris 300 mM NaCl, pH 8 were collected on the BM29 beam line at the ESRF storage ring (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.4 m and at a wavelength of λ = 0.93 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 0.8 and 15 mg/ml were measured . 10 successive 20 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.

Solution scattering data of Aureochrome1a- A´α-LOV-Jα in the light state (blue light illuminated for 5-10 min). The measurement was performed at two concentration 42 and 833 micomolar concentration. The buffer-only scattering was subtracted and the data sets were merged to generate representative curve. The analysis was performed using the ATSAS package. The displayed model is an averaged and volume corrected dummy atom representation (DAMFILT). For further sequence information refer to the Joint Genome Institute (JGI) entry JGI-49116.

Aureochrome1a-A´α-LOV-Jα (Aureochrome1a-A)
Mol. type   Protein
Organism   Phaeodactylum tricornutum
Olig. state   Dimer
Mon. MW   18.0 kDa
Sequence   FASTA