Standard proteins

Darja Ruskule.

SASDAK2 – Myoglobin in PBS

Myoglobin
MWI(0) 11 kDa
MWexpected 17 kDa
VPorod 32 nm3
log I(s) 1.06×101 1.06×100 1.06×10-1 1.06×10-2
Myoglobin small angle scattering data  s, nm-1
ln I(s)
Myoglobin Guinier plot ln 1.06×101 Rg: 1.6 nm 0 (1.6 nm)-2 s2
(sRg)2I(s)/I(0)
Myoglobin Kratky plot 1.104 0 √3 sRg
p(r)
Myoglobin pair distance distribution function Rg: 1.7 nm 0 Dmax: 5 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Myoglobin PDB (PROTEIN DATA BANK) model
log I(s)
 s, nm-1
Myoglobin GASBOR model
Synchrotron SAXS data from solutions of Myoglobin in PBS in PBS, pH 7.4 were collected on the EMBL X33 beam line at the DORIS III, DESY storage ring (Hamburg, Germany) using a Pilatus 1M-W detector at a sample-detector distance of 2.7 m and at a wavelength of λ = 0.15 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 25.00 mg/ml was measured at 10°C. Eight successive 15 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.

Concentration min = UNKNOWN

Tags: waxs X33
Myoglobin
Mol. type   Protein
Organism   Equus caballus
Olig. state   Monomer
Mon. MW   17.1 kDa
 
UniProt   P68082
Sequence   FASTA
 
PDB ID   1WLA