Structure of a Native-like Aureochrome 1a LOV Domain Dimer from Phaeodactylum tricornutum.

Banerjee A, Herman E, Kottke T, Essen LO, Structure 24(1):171-178 (2016) Europe PMC

SASDAK8 – Dark state solution structure of Aureochrome1a- A´α-LOV-Jα

Aureochrome1a-A´α-LOV-Jα
MWexperimental 43 kDa
MWexpected 36 kDa
VPorod 67 nm3
log I(s) 4.23×101 4.23×100 4.23×10-1 4.23×10-2
Aureochrome1a-A´α-LOV-Jα small angle scattering data  s, nm-1
ln I(s)
Aureochrome1a-A´α-LOV-Jα Guinier plot ln 4.23×101 Rg: 3.2 nm 0 (3.2 nm)-2 s2
(sRg)2I(s)/I(0)
Aureochrome1a-A´α-LOV-Jα Kratky plot 1.104 0 √3 sRg
p(r)
Aureochrome1a-A´α-LOV-Jα pair distance distribution function Rg: 3.3 nm 0 Dmax: 13 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Aureochrome1a-A´α-LOV-Jα DAMMIF model
Synchrotron SAXS data from solutions of Dark state solution structure of Aureochrome1a- A´α-LOV-Jα in 10 mM Tris 300 mM NaCl, pH 8 were collected on the BM29 beam line at the ESRF storage ring (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.4 m and at a wavelength of λ = 0.93 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 0.8 and 15 mg/ml were measured . 10 successive 20 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Solution scattering of N-terminally His6 tagged A´α-LOV-Jα was measured in the dark state (under safe red light). Two concentrations of the sample were measured at 40 and 833 micromolar and then merged. Radiation damage was observed at very low extent. The data were normalized and the buffer only scattering subtracted. The analysis was performed using ATSAS. The displayed model is an averaged and volume corrected dummy atom representation (DAMFILT). For further sequence information refer to the Joint Genome Institute (JGI) entry JGI-49116.

Aureochrome1a-A´α-LOV-Jα (Aureochrome1a-A)
Mol. type   Protein
Organism   Phaeodactylum tricornutum
Olig. state   Dimer
Mon. MW   18.0 kDa
Sequence   FASTA