Structure and catalytic mechanism of the evolutionarily unique bacterial chalcone isomerase.

Thomsen M, Tuukkanen A, Dickerhoff J, Palm GJ, Kratzat H, Svergun DI, Weisz K, Bornscheuer UT, Hinrichs W, Acta Crystallogr D Biol Crystallogr 71(Pt 4):907-17 (2015) Europe PMC

SASDAN6 – Chalcone isomerase, CHI_Δlid construct

Chalcone isomerase deltaLid
MWI(0) 150 kDa
MWexpected 181 kDa
VPorod 270 nm3
log I(s) 2.72×104 2.72×103 2.72×102 2.72×101
Chalcone isomerase deltaLid small angle scattering data  s, nm-1
ln I(s)
Chalcone isomerase deltaLid Guinier plot ln 2.73×104 Rg: 3.6 nm 0 (3.6 nm)-2 s2
(sRg)2I(s)/I(0)
Chalcone isomerase deltaLid Kratky plot 1.104 0 √3 sRg
p(r)
Chalcone isomerase deltaLid pair distance distribution function Rg: 3.5 nm 0 Dmax: 11 nm

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of Chalcone isomerase, CHI_Δlid construct in 50 mM sodium phosphate, pH 6.8 were collected on the EMBL P12 beam line at the PETRA III storage ring (DESY; Hamburg, Germany) using a Pilatus 2M detector at a sample-detector distance of 3.1 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 1.3 and 9.8 mg/ml were measured at 10°C. 20 successive 0.050 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.

CHI_Δlid deletion mutant is missing amino acid residues His109 - Ala130.

Chalcone isomerase deltaLid (CHI_Δlid)
Mol. type   Protein
Organism   Eubacterium ramulus
Olig. state   Hexamer
Mon. MW   30.2 kDa
 
UniProt   V9P0A9