KSHV but not MHV-68 LANA induces a strong bend upon binding to terminal repeat viral DNA.

Ponnusamy R, Petoukhov MV, Correia B, Custodio TF, Juillard F, Tan M, Pires de Miranda M, Carrondo MA, Simas JP, Kaye KM, Svergun DI, McVey CE, Nucleic Acids Res 43(20):10039-54 (2015) Europe PMC

SASDAN8 – mLBS1-2 DNA

MHV-68 TR DNA

MW^experimental	30	kDa
MW^expected	30	kDa
V^Porod	50	nm³

log I(s) 1.78×10³ 1.78×10² 1.78×10¹ 1.78×10⁰

MHV-68 TR DNA small angle scattering data

s, nm^-1

Log plot Log-Log plot

ln I(s)

ln 1.79×10³ R_g: 4 nm 0 (4 nm)^-2 s²

(sR_g)²I(s)/I(0)

1.104 0 √3 sR_g

p(r)	0 D_max: 16 nm

Data validation

Experimental data range

p(r) fit to data

Metric

Value

s_min D_max / π

1.2

N_Shannon

Worse

Better

Metric

Value

p_cormap

χ²

0.422
1.088

Worse

Better

Fits and models

log I(s)	s, nm^-1
+3 Δ/σ -3	s, nm^-1

Synchrotron SAXS data from solutions of mLBS1-2 DNA in 10 mM TRIS 150 mM NaCl, pH 7.6 were collected on the EMBL P12 beam line at the PETRA III storage ring (DESY; Hamburg, Germany) using a Pilatus 2M detector at a sample-detector distance of 3.1 m and at a wavelength of λ = 0.12 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 5.00 mg/ml was measured at 10°C. 20 successive 0.050 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Study of oligomeric states of mLANA and kLANA and their complexes with DNA

MHV-68 TR DNA (mLBS1-2 )
Mol. type		DNA
Organism		unidentified herpesvirus
Olig. state		Monomer
Mon. MW		30 kDa