| MWexperimental | 340 | kDa |
| MWexpected | 381 | kDa |
| VPorod | 700 | nm3 |
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log I(s)
2.74×102
2.74×101
2.74×100
2.74×10-1
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s, nm-1
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Using stable MutS dimers and tetramers to quantitatively analyze DNA mismatch recognition and sliding clamp formation.
Groothuizen FS, Fish A, Petoukhov MV, Reumer A, Manelyte L, Winterwerp HH, Marinus MG, Lebbink JH, Svergun DI, Friedhoff P, Sixma TK, Nucleic Acids Res 41(17):8166-81 (2013) Europe PMCSASDAQ3 – MutS tetramer
DNA mismatch repair protein MutS|
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Fits and models
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Synchrotron SAXS
data from solutions of
MutS tetramer
in
50 mM HEPES 50 mM KCl, pH 7.5
were collected
on the
EMBL X33 beam line
at the DORIS III, DESY storage ring
(Hamburg, Germany)
using a Pilatus 2M detector
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
Solute concentrations ranging between 0.7 and 4.9 mg/ml were measured
at 10°C.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.
Wavelength = UNKNOWN. Sample detector distance = UNKNOWN. X-ray Exposure time = UNKNOWN. Number of frames = UNKNOWN
Tags:
X33
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