The flexibility of a homeodomain transcription factor heterodimer and its allosteric regulation by DNA binding.

Mathiasen L, Valentini E, Boivin S, Cattaneo A, Blasi F, Svergun DI, Bruckmann C, FEBS J 283(16):3134-54 (2016) Europe PMC

SASDAQ7 – PBX1-PREB1-DNA complex

Pre-B-cell leukemia transcription factor 1
Homeobox protein PKNOX1
DNA 22mer
MWI(0) 80 kDa
MWexpected 85 kDa
VPorod 173 nm3
log I(s) 1.64×103 1.64×102 1.64×101 1.64×100
Pre-B-cell leukemia transcription factor 1 Homeobox protein PKNOX1 DNA 22mer small angle scattering data  s, nm-1
ln I(s)
Pre-B-cell leukemia transcription factor 1 Homeobox protein PKNOX1 DNA 22mer Guinier plot ln 1.64×103 Rg: 4.8 nm 0 (4.8 nm)-2 s2
(sRg)2I(s)/I(0)
Pre-B-cell leukemia transcription factor 1 Homeobox protein PKNOX1 DNA 22mer Kratky plot 1.104 0 3 sRg
p(r)
Pre-B-cell leukemia transcription factor 1 Homeobox protein PKNOX1 DNA 22mer pair distance distribution function Rg: 4.8 nm 0 Dmax: 16.3 nm

Data validation


Fits and models


log I(s)
 s, nm-1
Pre-B-cell leukemia transcription factor 1 Homeobox protein PKNOX1 DNA 22mer DAMMIF model

log I(s)
 s, nm-1
Pre-B-cell leukemia transcription factor 1 Homeobox protein PKNOX1 DNA 22mer MONSA model

log I(s)
 s, nm-1

Synchrotron SAXS data from solutions of PBX1-PREB1-DNA complex in 20 mM Tris-HCl 150 mM NaCl 5% glycerol 1 mM DTT, pH 7.4 were collected on the EMBL P12 beam line at the PETRA III storage ring (DESY; Hamburg, Germany) using a Pilatus 2M detector at a sample-detector distance of 3.1 m and at a wavelength of λ = 0.12 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 3.80 mg/ml was measured. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Cell temperature = UNKNOWN. Storage temperature = UNKNOWN. X-ray Exposure time = UNKNOWN. Number of frames = UNKNOWN

Pre-B-cell leukemia transcription factor 1 (PBX1)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   34.5 kDa
 
UniProt   P40424 (1-308)
Sequence   FASTA
 
Homeobox protein PKNOX1 (PREP1)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   38.3 kDa
 
UniProt   P55347 (1-344)
Sequence   FASTA
 
DNA 22mer
Mol. type   DNA
Olig. state   Monomer
Mon. MW   11.9 kDa
Sequence   FASTA