Standard proteins

Darja Ruskule.

SASDAR2 – Bovine pancreatic ribonuclease A in PBS

Ribonuclease pancreatic
MWI(0) 14 kDa
MWexpected 16 kDa
VPorod 17 nm3
log I(s) 1.36×101 1.36×100 1.36×10-1 1.36×10-2
Ribonuclease pancreatic small angle scattering data  s, nm-1
ln I(s)
Ribonuclease pancreatic Guinier plot ln 1.37×101 Rg: 1.6 nm 0 (1.6 nm)-2 s2
(sRg)2I(s)/I(0)
Ribonuclease pancreatic Kratky plot 1.104 0 √3 sRg
p(r)
Ribonuclease pancreatic pair distance distribution function Rg: 1.6 nm 0 Dmax: 5 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Ribonuclease pancreatic DAMMIF model
log I(s)
 s, nm-1
Ribonuclease pancreatic PDB (PROTEIN DATA BANK) model
Synchrotron SAXS data from solutions of Bovine pancreatic ribonuclease A in PBS in PBS, pH 7.4 were collected on the EMBL X33 beam line at the DORIS III, DESY storage ring (Hamburg, Germany) using a Pilatus 1M-W detector at a sample-detector distance of 2.7 m and at a wavelength of λ = 0.15 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 6.4 and 22.8 mg/ml were measured at 10°C. Eight successive 15 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentrations were extrapolated to infinite dilution and merged with the higher concentration data to yield the final composite scattering curve.

Tags: X33
Ribonuclease pancreatic (RNase)
Mol. type   Protein
Organism   Bos taurus
Olig. state   Monomer
Mon. MW   16.5 kDa
 
UniProt   P61823
Sequence   FASTA
 
PDB ID   1C0B