| MWexperimental | 18 | kDa |
| MWexpected | 22 | kDa |
| VPorod | 37 | nm3 |
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log I(s)
1.67×101
1.67×100
1.67×10-1
1.67×10-2
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s, nm-1
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The basic keratin 10-binding domain of the virulence-associated pneumococcal serine-rich protein PsrP adopts a novel MSCRAMM fold.
Schulte T, Löfling J, Mikaelsson C, Kikhney A, Hentrich K, Diamante A, Ebel C, Normark S, Svergun D, Henriques-Normark B, Achour A, Open Biol 4:130090 (2014) Europe PMCSASDAR3 – PsrP functional binding region
Functional binding region (187-385) of the pneumococcal serine-rich repeat protein|
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Fits and models
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Rg, nm
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Synchrotron SAXS
data from solutions of
PsrP functional binding region
in
20 mM sodium citrate 250 mM NaCl 2.5 % Glycerol, pH 5.5
were collected
on the
EMBL X33 beam line
at the DORIS III, DESY storage ring
(Hamburg, Germany)
using a Pilatus 1M-W detector
at a sample-detector distance of 2.7 m and
at a wavelength of λ = 0.15 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
Solute concentrations ranging between 1.1 and 8.7 mg/ml were measured
at 10°C.
Eight successive
15 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.
Tags:
X33
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