Intrinsic structural disorder of mouse proNGF.

Paoletti F, Covaceuszach S, Konarev PV, Gonfloni S, Malerba F, Schwarz E, Svergun DI, Cattaneo A, Lamba D, Proteins 75(4):990-1009 (2009) Europe PMC

SASDAR5 – ProNGF

ProNGF
MWI(0) 44 kDa
MWexpected 45 kDa
VPorod 92 nm3
log I(s) 1.23×102 1.23×101 1.23×100 1.23×10-1
ProNGF small angle scattering data  s, nm-1
ln I(s)
ProNGF Guinier plot ln 1.23×102 Rg: 2.6 nm 0 (2.6 nm)-2 s2
(sRg)2I(s)/I(0)
ProNGF Kratky plot 1.104 0 √3 sRg
p(r)
ProNGF pair distance distribution function Rg: 2.6 nm 0 Dmax: 8 nm

Data validation

Fits and models

log I(s)
 s, nm-1
ProNGF DAMMIN model
log I(s)
 s, nm-1
ProNGF BUNCH model
Synchrotron SAXS data from solutions of ProNGF in 50 mM Naphosphat 0.5 M Ammonium Sulfate(NH4)2SO4, pH 7 were collected on the EMBL X33 beam line at the DORIS III, DESY storage ring (Hamburg, Germany) using a MAR 345 Image Plate detector (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 3.00 mg/ml was measured at 15°C. Two successive 120 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Wavelength = UNKNOWN. Sample detector distance = UNKNOWN

Tags: X33
ProNGF (ProNGF)
Mol. type   Protein
Organism   Mouse submandibulary glands
Olig. state   Dimer
Mon. MW   22.5 kDa
Sequence   FASTA