The flexibility of a homeodomain transcription factor heterodimer and its allosteric regulation by DNA binding.

Mathiasen L, Valentini E, Boivin S, Cattaneo A, Blasi F, Svergun DI, Bruckmann C, FEBS J 283(16):3134-54 (2016) Europe PMC

SASDAR7 – DNA 22mer

DNA 22mer
MWexperimental 13 kDa
MWexpected 12 kDa
VPorod 19 nm3
log I(s) 1.26×103 1.26×102 1.26×101 1.26×100
DNA 22mer small angle scattering data  s, nm-1
ln I(s)
DNA 22mer Guinier plot ln 1.26×103 Rg: 2.2 nm 0 (2.2 nm)-2 s2
(sRg)2I(s)/I(0)
DNA 22mer Kratky plot 1.104 0 √3 sRg
p(r)
DNA 22mer pair distance distribution function Rg: 2.3 nm 0 Dmax: 7.5 nm

Data validation

Fits and models

log I(s)
 s, nm-1
DNA 22mer DAMMIF model
Synchrotron SAXS data from solutions of DNA 22mer in 20 mM Tris-HCl 150 mM NaCl 5% glycerol 1 mM DTT, pH 7.4 were collected on the EMBL P12 beam line at the PETRA III storage ring (DESY; Hamburg, Germany) using a Pilatus 2M detector at a sample-detector distance of 3.1 m and at a wavelength of λ = 0.12 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 1.50 mg/ml was measured. 20 successive 0.050 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Cell temperature = UNKNOWN. Storage temperature = UNKNOWN

DNA 22mer
Mol. type   DNA
Olig. state   Monomer
Mon. MW   11.9 kDa
Sequence   FASTA