Olfactomedin-1 Has a V-shaped Disulfide-linked Tetrameric Structure.

Pronker MF, Bos TG, Sharp TH, Thies-Weesie DM, Janssen BJ, J Biol Chem 290(24):15092-101 (2015) Europe PMC

SASDAS7 – mouse olfactomedin-1

Noelin
MWexperimental 249 kDa
MWexpected 256 kDa
VPorod 616 nm3
log I(s) 2.49×102 2.49×101 2.49×100 2.49×10-1
Noelin small angle scattering data  s, nm-1
ln I(s)
Noelin Guinier plot ln 2.49×102 Rg: 8.5 nm 0 (8.5 nm)-2 s2
(sRg)2I(s)/I(0)
Noelin Kratky plot 1.104 0 √3 sRg
p(r)
Noelin pair distance distribution function Rg: 8.8 nm 0 Dmax: 30 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Noelin DAMMIF model
Synchrotron SAXS data from solutions of mouse olfactomedin-1 in 20 mM HEPES 150 mM NaCl, pH 7.5 were collected on the BM29 beam line at the ESRF storage ring (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.4 m and at a wavelength of λ = 0.93 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 3.55 mg/ml was measured at 20°C. 18 successive 0.056 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

SAXS was used to determine that Olfactomedin-1 forms tetramers in solution (confirmed by SEC-MALS and AUC). Scattering data were combined with crystallographic and negative stain electron tomography data to construct a model of a V-shaped dimer-of-dimers.

Noelin (Olfactomedin-1)
Mol. type   Protein
Organism   Mus musculus
Olig. state   Tetramer
Mon. MW   64 kDa
 
UniProt   O88998
Sequence   FASTA