Atomic structures of two novel immunoglobulin-like domain pairs in the actin cross-linking protein filamin.

Heikkinen OK, Ruskamo S, Konarev PV, Svergun DI, Iivanainen T, Heikkinen SM, Permi P, Koskela H, Kilpeläinen I, Ylänne J, J Biol Chem 284(37):25450-8 (2009) Europe PMC

SASDAT3 – Fila18-19

immunoglobulin- like filamin two-domain fragment 18-19
MWexperimental 17 kDa
MWexpected 20 kDa
VPorod 34 nm3
log I(s) 9.83×101 9.83×100 9.83×10-1 9.83×10-2
immunoglobulin- like filamin two-domain fragment 18-19 small angle scattering data  s, nm-1
ln I(s)
immunoglobulin- like filamin two-domain fragment 18-19 Guinier plot ln 9.83×101 Rg: 2.1 nm 0 (2.1 nm)-2 s2
(sRg)2I(s)/I(0)
immunoglobulin- like filamin two-domain fragment 18-19 Kratky plot 1.104 0 √3 sRg
p(r)
immunoglobulin- like filamin two-domain fragment 18-19 pair distance distribution function Rg: 2.1 nm 0 Dmax: 6.5 nm

Data validation

Fits and models

log I(s)
 s, nm-1
immunoglobulin- like filamin two-domain fragment 18-19 GASBOR model
Synchrotron SAXS data from solutions of Fila18-19 in 100 mM NaCl 10 mM dithiothreitol 20 mM Tris, pH 8 were collected on the EMBL X33 beam line at the DORIS III, DESY storage ring (Hamburg, Germany) using a MAR 345 Image Plate detector (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). . The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Wavelength = UNKNOWN. Cell temperature = UNKNOWN. Storage temperature = UNKNOWN. Sample detector distance = UNKNOWN. X-ray Exposure time = UNKNOWN. Number of frames = UNKNOWN. Concentration = UNKNOWN

Tags: X33
immunoglobulin- like filamin two-domain fragment 18-19 (Fila18-19)
Mol. type   Protein
Organism   Escherichia coli
Olig. state   Monomer
Mon. MW   20.4 kDa
Sequence   FASTA