Atomic structures of two novel immunoglobulin-like domain pairs in the actin cross-linking protein filamin.

Heikkinen OK, Ruskamo S, Konarev PV, Svergun DI, Iivanainen T, Heikkinen SM, Permi P, Koskela H, Kilpeläinen I, Ylänne J, J Biol Chem 284(37):25450-8 (2009) Europe PMC

SASDAU3 – Fila22-23

immunoglobulin- like filamin two-domain fragment 22-23
MWexperimental 24 kDa
MWexpected 19 kDa
VPorod 32 nm3
log I(s) 3.43×101 3.43×100 3.43×10-1 3.43×10-2
immunoglobulin- like filamin two-domain fragment 22-23 small angle scattering data  s, nm-1
ln I(s)
immunoglobulin- like filamin two-domain fragment 22-23 Guinier plot ln 3.43×101 Rg: 2.8 nm 0 (2.8 nm)-2 s2
(sRg)2I(s)/I(0)
immunoglobulin- like filamin two-domain fragment 22-23 Kratky plot 1.104 0 √3 sRg
p(r)
immunoglobulin- like filamin two-domain fragment 22-23 pair distance distribution function Rg: 2.8 nm 0 Dmax: 9 nm

Data validation

Fits and models

log I(s)
 s, nm-1
immunoglobulin- like filamin two-domain fragment 22-23 GASBOR model
Synchrotron SAXS data from solutions of Fila22-23 in 100 mM NaCl 10 mM dithiothreitol 20 mM Tris, pH 8 were collected on the EMBL X33 beam line at the DORIS III, DESY storage ring (Hamburg, Germany) using a MAR 345 Image Plate detector (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). . The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Wavelength = UNKNOWN. Cell temperature = UNKNOWN. Storage temperature = UNKNOWN. Sample detector distance = UNKNOWN. X-ray Exposure time = UNKNOWN. Number of frames = UNKNOWN. Concentration = UNKNOWN

Tags: X33
immunoglobulin- like filamin two-domain fragment 22-23 (Fila22-23)
Mol. type   Protein
Organism   Escherichia coli
Olig. state   Monomer
Mon. MW   18.7 kDa
Sequence   FASTA