Endophilin-A1 BAR domain interaction with arachidonyl CoA.

Petoukhov MV, Weissenhorn W, Svergun DI, Front Mol Biosci 1:20 (2014) Europe PMC

SASDAX5 – Endophilin-CoA complex

Endophilin-A1 BAR domain
arachidonyl-CoA
MWexperimental 130 kDa
MWexpected 118 kDa
VPorod 480 nm3
log I(s) 4.35×102 4.35×101 4.35×100 4.35×10-1
Endophilin-A1 BAR domain arachidonyl-CoA small angle scattering data  s, nm-1
ln I(s)
Endophilin-A1 BAR domain arachidonyl-CoA Guinier plot ln 4.35×102 Rg: 5.9 nm 0 (5.9 nm)-2 s2
(sRg)2I(s)/I(0)
Endophilin-A1 BAR domain arachidonyl-CoA Kratky plot 1.104 0 √3 sRg
Dmax: 19 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Endophilin-A1 BAR domain arachidonyl-CoA SASREF model
Synchrotron SAXS data from solutions of Endophilin-CoA complex in 50 mM TRIS-HCL 300 mM NaCl, pH 8 were collected on the EMBL X33 beam line at the DORIS III, DESY storage ring (Hamburg, Germany) using a MAR 345 Image Plate detector (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 1.00 mg/ml was measured. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Wavelength = UNKNOWN. Cell temperature = UNKNOWN. Storage temperature = UNKNOWN. Sample detector distance = UNKNOWN. X-ray Exposure time = UNKNOWN. Number of frames = UNKNOWN

Tags: X33
Endophilin-A1 BAR domain (Endophilin)
Mol. type   Protein
Organism   Mus musculus
Olig. state   Dimer
Mon. MW   29 kDa
Sequence   FASTA
 
arachidonyl-CoA (CoA)
Mol. type   Other
Olig. state   Other