Structure and target interaction of a G-quadruplex RNA-aptamer.

Szameit K, Berg K, Kruspe S, Valentini E, Magbanua E, Kwiatkowski M, Chauvot de Beauchêne I, Krichel B, Schamoni K, Uetrecht C, Svergun DI, Schlüter H, Zacharias M, Hahn U, RNA Biol 13(10):973-987 (2016) Europe PMC

SASDAX7 – Aptamer AIR-3A G18U

AIR-3A G18U
MWexperimental 24 kDa
MWexpected 25 kDa
VPorod 24 nm3
log I(s) 3.20×103 3.20×102 3.20×101 3.20×100
AIR-3A G18U small angle scattering data  s, nm-1
ln I(s)
AIR-3A G18U Guinier plot ln 3.20×103 Rg: 2.5 nm 0 (2.5 nm)-2 s2
(sRg)2I(s)/I(0)
AIR-3A G18U Kratky plot 1.104 0 √3 sRg
p(r)
AIR-3A G18U pair distance distribution function Rg: 2.5 nm 0 Dmax: 8.3 nm

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of Aptamer AIR-3A G18U in water, pH 7.5 were collected on the EMBL P12 beam line at the PETRA III storage ring (DESY; Hamburg, Germany) using a Pilatus 2M detector at a sample-detector distance of 3.1 m and at a wavelength of λ = 0.12 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 0.20 mg/ml was measured at 10°C. 20 successive 0.050 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

AIR-3A G18U
Mol. type   RNA
Olig. state   Tetramer
Mon. MW   6.3 kDa
Sequence   FASTA