Endophilin-A1 BAR domain interaction with arachidonyl CoA.

Petoukhov MV, Weissenhorn W, Svergun DI, Front Mol Biosci 1:20 (2014) Europe PMC

SASDAY5 – Free endophilin

Endophilin-A1 BAR domain
MWexperimental 50 kDa
MWexpected 58 kDa
VPorod 90 nm3
log I(s) 7.10×101 7.10×100 7.10×10-1 7.10×10-2
Endophilin-A1 BAR domain small angle scattering data  s, nm-1
ln I(s)
Endophilin-A1 BAR domain Guinier plot ln 7.11×101 Rg: 3.3 nm 0 (3.3 nm)-2 s2
(sRg)2I(s)/I(0)
Endophilin-A1 BAR domain Kratky plot 1.104 0 √3 sRg
Dmax: 13.5 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Endophilin-A1 BAR domain CORAL model
Synchrotron SAXS data from solutions of Free endophilin in 50 mM TRIS-HCL 300 mM NaCl, pH 8 were collected on the EMBL X33 beam line at the DORIS III, DESY storage ring (Hamburg, Germany) using a MAR 345 Image Plate detector (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). . The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.

Wavelength = UNKNOWN. Cell temperature = UNKNOWN. Storage temperature = UNKNOWN. Sample detector distance = UNKNOWN. X-ray Exposure time = UNKNOWN. Number of frames = UNKNOWN. Concentration min = UNKNOWN. Concentration max = UNKNOWN

Tags: X33
Endophilin-A1 BAR domain (Endophilin)
Mol. type   Protein
Organism   Mus musculus
Olig. state   Dimer
Mon. MW   29 kDa
Sequence   FASTA