| MWexperimental | 143 | kDa |
| MWexpected | 246 | kDa |
| VPorod | 240 | nm3 |
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log I(s)
2.50×10-2
2.50×10-3
2.50×10-4
2.50×10-5
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s, nm-1
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Structure and target interaction of a G-quadruplex RNA-aptamer.
Szameit K, Berg K, Kruspe S, Valentini E Magbanua E, Kwiatkowski M, Chauvot de Beauchêne I, Krichel B, Schamoni K, Uetrecht C, Svergun DI, Schlüter H, Zacharias M, Hahn U, RNA Biol 13(10):973-987 (2016) Europe PMCSASDAY7 – IL-6R tetramer-dimer equilibrium
Interleukin-6 receptor subunit alphaInterleukin-6 receptor subunit alpha
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Fits and models
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Synchrotron SAXS
data from solutions of
IL-6R tetramer-dimer equilibrium
in
water, pH 7.5
were collected
on the
B21 beam line
at the Diamond Light Source storage ring
(Didcot, UK)
using a Pilatus 2M detector
at a sample-detector distance of 3.9 m and
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
One solute concentration of 0.10 mg/ml was measured.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
The curve was fitted with a tetramer obtained with CORAL from the unit cell tetrameric structure of the pdb code 1N26 with a Chi-squared-value of 2.49. Since the experimental MW appeared smaller than a tetramer (confirmed by a not-fitting tetrameric model) we tried to fit a mixture of tetramer-dimer using OLIGOMER. Two possible dimers were possible: a compact and an extended one. With the compact dimer mixture OLIGOMER estimated a volume fraction of 65% tetramer and 35% dimer and a corresponding Chi-squared-value of 1.92. With the extended dimer mixture OLIGOMER estimated a volume fraction of 47% tetramer and 53% dimer and a improved Chi-squared-value of 1.33. |
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