| MWexperimental | 143 | kDa |
| MWexpected | 246 | kDa |
| VPorod | 240 | nm3 |
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log I(s)
2.50×10-2
2.50×10-3
2.50×10-4
2.50×10-5
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s, nm-1
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Structure and target interaction of a G-quadruplex RNA-aptamer.
Szameit K, Berg K, Kruspe S, Valentini E, Magbanua E, Kwiatkowski M, Chauvot de Beauchêne I, Krichel B, Schamoni K, Uetrecht C, Svergun DI, Schlüter H, Zacharias M, Hahn U, RNA Biol 13(10):973-987 (2016) Europe PMCSASDAY7 – IL-6R tetramer-dimer equilibrium
Interleukin-6 receptor subunit alphaInterleukin-6 receptor subunit alpha
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Fits and models
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Synchrotron SAXS
data from solutions of
IL-6R tetramer-dimer equilibrium
in
water, pH 7.5
were collected
on the
B21 beam line
at the Diamond Light Source storage ring
(Didcot, UK)
using a Pilatus 2M detector
at a sample-detector distance of 3.9 m and
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
One solute concentration of 0.10 mg/ml was measured.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
The curve was fitted with a tetramer obtained with CORAL from the unit cell tetrameric structure of the pdb code 1N26 with a Chi-squared-value of 2.49. Since the experimental MW appeared smaller than a tetramer (confirmed by a not-fitting tetrameric model) we tried to fit a mixture of tetramer-dimer using OLIGOMER. Two possible dimers were possible: a compact and an extended one. With the compact dimer mixture OLIGOMER estimated a volume fraction of 65% tetramer and 35% dimer and a corresponding Chi-squared-value of 1.92. With the extended dimer mixture OLIGOMER estimated a volume fraction of 47% tetramer and 53% dimer and a improved Chi-squared-value of 1.33. |
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