Dystrophin's central domain forms a complex filament that becomes disorganized by in-frame deletions.

Delalande O, Molza AE, Dos Santos Morais R, Chéron A, Pollet É, Raguenes-Nicol C, Tascon C, Giudice E, Guilbaud M, Nicolas A, Bondon A, Leturcq F, Férey N, Baaden M, Perez J, Roblin P, Piétri-Rouxel F, Hubert JF, Czjzek M, Le Rumeur E, J Biol Chem 293(18):6637-6646 (2018) Europe PMC

SASDB43 – Human dystrophin central domain repeats 1 to 2

Dystrophin central domain repeats 1 to 2
MWexperimental 26 kDa
MWexpected 26 kDa
VPorod 42 nm3
log I(s) 3.84×10-2 3.84×10-3 3.84×10-4 3.84×10-5
Dystrophin central domain repeats 1 to 2 small angle scattering data  s, nm-1
ln I(s)
Dystrophin central domain repeats 1 to 2 Guinier plot ln 3.84×10-2 Rg: 3.0 nm 0 (3.0 nm)-2 s2
(sRg)2I(s)/I(0)
Dystrophin central domain repeats 1 to 2 Kratky plot 1.104 0 √3 sRg
p(r)
Dystrophin central domain repeats 1 to 2 pair distance distribution function Rg: 3.1 nm 0 Dmax: 10.6 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Dystrophin central domain repeats 1 to 2 NONE model
Synchrotron SAXS data were measured from solutions of Human Dystrophin central domain repeats 1 to 2 (amino acids 338-563) in 20 mM Tris 150 mM NaCl, 1 mM EDTA, 2% glycerol buffer using size exclusion chromatography (SEC-SAXS) in combination with UV spectroscopy on the SWING beam line (SOLEIL, Saint-Aubin, France). The scattering data, I(s) vs s (s = 4π sin θ/λ, where 2θ is the scattering angle) were collected through the corresponding SEC protein elution peak (20 x 60 s frames) as well as from the SEC column running buffer. The buffer scattering contributions were subtracted from each sample frame, which were then scaled and averaged to produce the SAXS profile displayed in this entry. The molecular weight estimate (MW) are derived from the Porod volume, Vp, where MW is approximately Vp/1.6. The SEC Rg and I(0) and UV trace are included in the additional files to this entry.

Cell temperature = UNKNOWN. Number of frames = UNKNOWN. Concentration = UNKNOWN

Dystrophin central domain repeats 1 to 2 (Dystrophin 338-563)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   26.4 kDa
 
UniProt   P11532 (338-563)
Sequence   FASTA