Nucleoplasmin-like domain of FKBP39 from Drosophila melanogaster forms a tetramer with partly disordered tentacle-like C-terminal segments.

Kozłowska M, Tarczewska A, Jakób M, Bystranowska D, Taube M, Kozak M, Czarnocki-Cieciura M, Dziembowski A, Orłowski M, Tkocz K, Ożyhar A, Sci Rep 7:40405 (2017) Europe PMC

SASDB47 – Full length tetrameric FKBP39 protein from Drosophila melanogaster

39 kDa FK506-binding nuclear protein
MWI(0) 161 kDa
MWexpected 163 kDa
VPorod 275 nm3
log I(s) 5.20×10-2 5.20×10-3 5.20×10-4 5.20×10-5
39 kDa FK506-binding nuclear protein small angle scattering data  s, nm-1
ln I(s)
39 kDa FK506-binding nuclear protein Guinier plot ln 5.20×10-2 Rg: 5.7 nm 0 (5.7 nm)-2 s2
(sRg)2I(s)/I(0)
39 kDa FK506-binding nuclear protein Kratky plot 1.104 0 √3 sRg
p(r)
39 kDa FK506-binding nuclear protein pair distance distribution function Rg: 5.8 nm 0 Dmax: 22 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Full length tetrameric FKBP39 protein from Drosophila melanogaster Rg histogram Rg, nm
39 kDa FK506-binding nuclear protein EOM/RANCH model
39 kDa FK506-binding nuclear protein EOM/RANCH model
39 kDa FK506-binding nuclear protein EOM/RANCH model
39 kDa FK506-binding nuclear protein EOM/RANCH model
Synchrotron SAXS data from solutions of full length tetrameric FKBP39 protein from Drosophila melanogaster in 50 mM Na2HPO4, 150 mM NaCl, pH 7 were collected on the B21 beam line at the Diamond Light Source (Oxfordshire, UK), using a Pilatus 2M detector at a sample-detector distance of 3.9 m and at a wavelength of λ = 0.1 nm (I(s) vs s = 4π sin θ/λ, where 2θ is the scattering angle). One solute concentration of 3.00 mg/ml was measured at 10°C. 18 successive 10 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Modeling of the full length FKBP39 protein from low resolution structure was performed using Ensemble Optimization Method (EOM). EOM models were then processed by PD2 ca2main program to generate models that have replaced bead residues by C-alpha backbone. The molecular weight of the protein was estimated using I(0) value obtained for the CS domain of barley SGT1. Molecular weight obtained using MoW (Fischer et al) is 158 kDa. In addition, a zip file containing models of the final EOM ensemble as well as the final Rg and Dmax distributions are available in the full entry zip archive.

39 kDa FK506-binding nuclear protein (FKBP39)
Mol. type   Protein
Organism   Drosophila melanogaster
Olig. state   Tetramer
Mon. MW   40.8 kDa
 
UniProt   P54397 (1-357)
Sequence   FASTA