| MWI(0) | 13 | kDa |
| MWexpected | 16 | kDa |
| VPorod | 24 | nm3 |
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log I(s)
2.26×103
2.26×102
2.26×101
2.26×100
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s, nm-1
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Calcium-Driven Folding of RTX Domain β-Rolls Ratchets Translocation of RTX Proteins through Type I Secretion Ducts.
Bumba L, Masin J, Macek P, Wald T, Motlova L, Bibova I, Klimova N, Bednarova L, Veverka V, Kachala M, Svergun DI, Barinka C, Sebo P, Mol Cell 62(1):47-62 (2016) Europe PMCSASDB84 – CyaA Block V
Adenylate cyclase toxin Block V|
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Fits and models
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Synchrotron SAXS
data from solutions of
CyaA Block V
in
10 mM Tris HCl 150 mM NaCl 10 mM CaCl2, pH 8
were collected
on the
EMBL P12 beam line
at the PETRA III storage ring
(DESY; Hamburg, Germany)
using a Pilatus 2M detector
at a sample-detector distance of 3.1 m and
at a wavelength of λ = 0.12 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
Solute concentrations ranging between 1.3 and 5 mg/ml were measured
at 10°C.
20 successive
0.050 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.
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