Basic domain of telomere guardian TRF2 reduces D-loop unwinding whereas Rap1 restores it.

Necasová I, Janoušková E, Klumpler T, Hofr C, Nucleic Acids Res 45(21):12170-12180 (2017) Europe PMC

SASDB89 – Telomeric DNA duplex

telomere DNA duplex
MWexperimental 11 kDa
MWexpected 11 kDa
VPorod 12 nm3
log I(s) 9.40×10-2 9.40×10-3 9.40×10-4 9.40×10-5
telomere DNA duplex small angle scattering data  s, nm-1
ln I(s)
telomere DNA duplex Guinier plot ln 9.40×10-2 Rg: 1.6 nm 0 (1.6 nm)-2 s2
(sRg)2I(s)/I(0)
telomere DNA duplex Kratky plot 1.104 0 √3 sRg
p(r)
telomere DNA duplex pair distance distribution function Rg: 1.7 nm 0 Dmax: 5.7 nm

Data validation

Fits and models

log I(s)
 s, nm-1
telomere DNA duplex MONSA model
SAXS data from solutions of telomeric DNA duplex in 20 mM Tris-HCl, 50 mM LiCl, pH 7.5 were collected using a Rigaku BioSAXS-1000 instrument at CEITEC (Brno, Czech Republic) equipped with a Pilatus 100K detector at a sample-detector distance of 0.5 m (I(s) vs s, where = 4πsinθ/λ; 2θ is the scattering angle and λ = 0.154 nm). Six successive 3600 second frames were collected at a sample temperature of 4°C and a solute concentration of 1.4 mg/ml. The data were normalized to the intensity of the transmitted beam and radially averaged and the corresponding scattering from the solvent-blank was subtracted to produced the scattering profile displayed in this entry.

Refer to the related SASBDB entries SASDB79 and SASDC62.

telomere DNA duplex
Mol. type   DNA
Olig. state   Monomer
Mon. MW   10.5 kDa
Sequence   FASTA