Dystrophin's central domain forms a complex filament that becomes disorganized by in-frame deletions.

Delalande O, Molza AE, Dos Santos Morais R, Chéron A, Pollet É, Raguenes-Nicol C, Tascon C, Giudice E, Guilbaud M, Nicolas A, Bondon A, Leturcq F, Férey N, Baaden M, Perez J, Roblin P, Piétri-Rouxel F, Hubert JF, Czjzek M, Le Rumeur E, J Biol Chem 293(18):6637-6646 (2018) Europe PMC

SASDB93 – Human dystrophin central domain repeats 16 to 19

Dystrophin central domain repeats 16 to 19.
MWexperimental 44 kDa
MWexpected 50 kDa
VPorod 70 nm3
log I(s) 1.92×10-2 1.92×10-3 1.92×10-4 1.92×10-5
Dystrophin central domain repeats 16 to 19. small angle scattering data  s, nm-1
ln I(s)
Dystrophin central domain repeats 16 to 19. Guinier plot ln 1.92×10-2 Rg: 4.6 nm 0 (4.6 nm)-2 s2
(sRg)2I(s)/I(0)
Dystrophin central domain repeats 16 to 19. Kratky plot 1.104 0 √3 sRg
p(r)
Dystrophin central domain repeats 16 to 19. pair distance distribution function Rg: 5.0 nm 0 Dmax: 20 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Dystrophin central domain repeats 16 to 19. NONE model
Synchrotron SAXS data were measured from solutions of Human Dystrophin central domain repeats 16 to 19 (amino acids 1984-2420) in 20 mM Tris 150 mM NaCl, 1 mM EDTA, 2% glycerol, 5% acetonitrile buffer using size exclusion chromatography (SEC-SAXS) on the SWING beam line (SOLEIL, Saint-Aubin, France). The sample load concentration was 6 mg/mL. The scattering data, I(s) vs s (s = 4π sin θ/λ, where 2θ is the scattering angle) were collected through the corresponding SEC protein elution peak (23 x 1.5 s frames) as well as from the SEC column running buffer. The buffer scattering contributions were subtracted from each sample frame, which were then scaled and averaged to produce the SAXS profile displayed in this entry. The molecular weight estimate (MW) are derived from the Porod volume, Vp, where MW is approximately Vp/1.6. The SEC Rg and I(0) trace is included in the additional files to this entry.

Cell temperature = UNKNOWN. Concentration = UNKNOWN

Dystrophin central domain repeats 16 to 19. (Dystrophin 1994-2420)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   50.1 kDa
 
UniProt   P11532 (1994-2420)
Sequence   FASTA