The Structure of the Plakin Domain of Plectin Reveals an Extended Rod-like Shape.

Ortega E, Manso JA, Buey RM, Carballido AM, Carabias A, Sonnenberg A, de Pereda JM, J Biol Chem 291(36):18643-62 (2016) Europe PMC

SASDBA4 – Plakin domain fragment of Human plectin (central region spectrin repeats: SR3-SR4-SR5-SR6 and SH3)

Plakin domain fragment of Human plectin encompassing spectrin repeats SR3-SR4-SR5-SR6 and SH3
MWexperimental 46 kDa
MWexpected 53 kDa
VPorod 91 nm3
log I(s) 1.05×103 1.05×102 1.05×101 1.05×100
Plakin domain fragment of Human plectin encompassing spectrin repeats SR3-SR4-SR5-SR6 and SH3 small angle scattering data  s, nm-1
ln I(s)
Plakin domain fragment of Human plectin encompassing spectrin repeats SR3-SR4-SR5-SR6 and SH3 Guinier plot ln 1.05×103 Rg: 5.1 nm 0 (5.1 nm)-2 s2
(sRg)2I(s)/I(0)
Plakin domain fragment of Human plectin encompassing spectrin repeats SR3-SR4-SR5-SR6 and SH3 Kratky plot 1.104 0 √3 sRg
p(r)
Plakin domain fragment of Human plectin encompassing spectrin repeats SR3-SR4-SR5-SR6 and SH3 pair distance distribution function Rg: 5.4 nm 0 Dmax: 21 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Plakin domain fragment of Human plectin encompassing spectrin repeats SR3-SR4-SR5-SR6 and SH3 DAMMIF model
Synchrotron SAXS data from solutions of a plakin domain fragment from Human plectin (central region spectrin repeats SR3-SR4-SR5-SR6 and SH3) in 20 mM sodium phosphate, 150 mM NaCl, 5% v/v glycerol, 3 mM DTT were collected on the P12 beam line of Petra-III (Hamburg, Germany) using a Pilatus 2M detector (I(s) vs s; s = 4π sin θ/λ, where 2θ is the scattering angle and λ=0.124 nm). Different solute concentrations in the range 1.70-26.50 mg/ml were measured using an exposure time of 1 s (recorded as 20 x 0.050 s frames). The data were normalized to the intensity of the transmitted beam and radially averaged and the scattering from the matched solvent-blank was subtracted. The data were normalized to protein concentration and then extrapolated to infinite dilution to simulate a zero-concentration scattering curve.

Plakin domain fragment of Human plectin encompassing spectrin repeats SR3-SR4-SR5-SR6 and SH3 (Plectin)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   53.2 kDa
 
UniProt   Q15149-2 (543-1006)
Sequence   FASTA