Conformational plasticity of RepB, the replication initiator protein of promiscuous streptococcal plasmid pMV158.

Boer DR, Ruiz-Masó JA, Rueda M, Petoukhov MV, Machón C, Svergun DI, Orozco M, del Solar G, Coll M, Sci Rep 6:20915 (2016) Europe PMC

SASDBC3 – RepB, the replication initiator protein of a promiscuous Streptococcal plasmid pMV158

Replication initiator protein of a promiscuous streptococcal plasmid pMV158.
MWexperimental 177 kDa
VPorod 282 nm3
log I(s) 3.88×102 3.88×101 3.88×100 3.88×10-1
Replication initiator protein of a promiscuous streptococcal plasmid pMV158. small angle scattering data  s, nm-1
ln I(s)
Replication initiator protein of a promiscuous streptococcal plasmid pMV158. Guinier plot ln 3.88×102 Rg: 3.8 nm 0 (3.8 nm)-2 s2
(sRg)2I(s)/I(0)
Replication initiator protein of a promiscuous streptococcal plasmid pMV158. Kratky plot 1.104 0 3 sRg
p(r)
Replication initiator protein of a promiscuous streptococcal plasmid pMV158. pair distance distribution function 0 Dmax: 10.6 nm

Data validation


Fits and models


log I(s)
 s, nm-1
Replication initiator protein of a promiscuous streptococcal plasmid pMV158. NONE model
Replication initiator protein of a promiscuous streptococcal plasmid pMV158. NONE model
Replication initiator protein of a promiscuous streptococcal plasmid pMV158. NONE model
Replication initiator protein of a promiscuous streptococcal plasmid pMV158. NONE model

Synchrotron SAXS data from solutions of RepB, the replication initiator protein of a promiscuous Streptococcal plasmid pMV158 in 10 mM TRIS 5 mM EDTA 1.0 M KCl, pH 8.5 were collected on the EMBL X33 beam line at the DORIS III, DESY storage ring (Hamburg, Germany) using a MAR 345 Image Plate detector (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). . The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Wavelength = UNKNOWN. Cell temperature = UNKNOWN. Storage temperature = UNKNOWN. Sample detector distance = UNKNOWN. X-ray Exposure time = UNKNOWN. Number of frames = UNKNOWN. Concentration = UNKNOWN

Tags: X33
Replication initiator protein of a promiscuous streptococcal plasmid pMV158. (RepB)
Mol. type   Protein
Organism   Streptococcus sp.
Olig. state   Hexamer