Insights on the structural dynamics of Leishmania braziliensis Hsp90 molecular chaperone by small angle X-ray scattering.

Seraphim TV, Silva KP, Dores-Silva PR, Barbosa LR, Borges JC, Int J Biol Macromol 97:503-512 (2017) Europe PMC

SASDBF3 – Leishmania braziliensis heat shock protein 90 (Hsp90) N and M domains.

Leishmania braziliensis heat shock protein 90 (Hsp90) N and M domains
MWexperimental 67 kDa
MWexpected 62 kDa
VPorod 89 nm3
log I(s) 1.30×10-3 1.30×10-4 1.30×10-5 1.30×10-6
Leishmania braziliensis heat shock protein 90 (Hsp90) N and M domains small angle scattering data  s, nm-1
ln I(s)
Leishmania braziliensis heat shock protein 90 (Hsp90) N and M domains Guinier plot ln 1.30×10-3 Rg: 3.2 nm 0 (3.2 nm)-2 s2
(sRg)2I(s)/I(0)
Leishmania braziliensis heat shock protein 90 (Hsp90) N and M domains Kratky plot 1.104 0 √3 sRg
p(r)
Leishmania braziliensis heat shock protein 90 (Hsp90) N and M domains pair distance distribution function Rg: 3.2 nm 0 Dmax: 13 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Leishmania braziliensis heat shock protein 90 (Hsp90) N and M domains DAMMIN model
X-ray synchrotron radiation scattering data from solutions of Leishmania braziliensis heat shock protein 90 (Hsp90) N and M domains in 25 mM Tris 100 mM NaCl, 1 mM 2-mercaptoethanol were collected on the SAXS2 Beamline camera on the storage ring Brazilian Synchrotron Light Laboratory (Campinas, Brazil) using a MAR Image Plate detector (s = 4π sin θ/λ, where 2θ is the scattering angle). The data were normalized to the intensity of the transmitted beam and radially averaged. The scattering of the solvent-blank was subtracted. The low angle data collected were obtained from a single concentration scattering curve.

Wavelength = UNKNOWN. Cell temperature = UNKNOWN. Storage temperature = UNKNOWN. Sample detector distance = UNKNOWN. X-ray Exposure time = UNKNOWN. Number of frames = UNKNOWN. Concentration = UNKNOWN

Leishmania braziliensis heat shock protein 90 (Hsp90) N and M domains (L. brazilien.Hsp90NM)
Mol. type   Protein
Organism   Leishmania braziliensis
Olig. state   Monomer
Mon. MW   62 kDa
Sequence   FASTA