Wing phosphorylation is a major functional determinant of the Lrs14-type biofilm and motility regulator AbfR1 in Sulfolobus acidocaldarius.

Li L, Banerjee A, Bischof LF, Maklad HR, Hoffmann L, Henche AL, Veliz F, Bildl W, Schulte U, Orell A, Essen LO, Peeters E, Albers SV, Mol Microbiol 105(5):777-793 (2017) Europe PMC

SASDBG2 – Wild-type archaeal biofilm regulator 1 (ABfR1: Transcriptional regulator ArsR family).

Wild-type archaeal biofilm regulator 1 (ABfR1: Transcriptional regulator ArsR family).
MWexperimental 27 kDa
MWexpected 26 kDa
VPorod 47 nm3
log I(s) 2.67×101 2.67×100 2.67×10-1 2.67×10-2
Wild-type archaeal biofilm regulator 1 (ABfR1: Transcriptional regulator ArsR family). small angle scattering data  s, nm-1
ln I(s)
Wild-type archaeal biofilm regulator 1 (ABfR1: Transcriptional regulator ArsR family). Guinier plot ln 2.67×101 Rg: 2.6 nm 0 (2.6 nm)-2 s2
(sRg)2I(s)/I(0)
Wild-type archaeal biofilm regulator 1 (ABfR1: Transcriptional regulator ArsR family). Kratky plot 1.104 0 √3 sRg
p(r)
Wild-type archaeal biofilm regulator 1 (ABfR1: Transcriptional regulator ArsR family). pair distance distribution function Rg: 2.6 nm 0 Dmax: 9.8 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Wild-type archaeal biofilm regulator 1 (ABfR1: Transcriptional regulator ArsR family). DAMMIN model
X-ray synchrotron radiation scattering data from solutions of N terminal affinity tag cleaved (by thrombin) AbfR1 in 20mMHEPES pH7.5, 200mM NaCl were collected on the BM29 beam line on the storage ring ESRF (Grenoble, France) using a 2D Photon counting Pilatus 1M pixel detector (s = 4π sin θ/λ, where 2θ is the scattering angle). Different solute concentrations in the range 2.5-9.00 mg/mL were measured. 10 successive 20 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the different curves were scaled for protein concentration. The low angle data collected were merged with the highest concentration high angle data to yield the final composite scattering curve.The analysis was performed using the ATSAS package. The displayed model is an averaged and volume corrected dummy atom representation (DAMFILT) after imposing P2 symmetry.

Wild-type archaeal biofilm regulator 1 (ABfR1: Transcriptional regulator ArsR family). (ABfR1-WT)
Mol. type   Protein
Organism   Sulfolobus acidocaldarius
Olig. state   Dimer
Mon. MW   13.2 kDa
 
UniProt   Q4JBH1 (1-116)
Sequence   FASTA