Wing phosphorylation is a major functional determinant of the Lrs14-type biofilm and motility regulator AbfR1 in Sulfolobus acidocaldarius.

Li L, Banerjee A, Bischof LF, Maklad HR, Hoffmann L, Henche AL, Veliz F, Bildl W, Schulte U, Orell A, Essen LO, Peeters E, Albers SV, Mol Microbiol 105(5):777-793 (2017) Europe PMC

SASDBH2 – Mutant archaeal biofilm regulator 1 (ABfR1) phosphomimic Y84E S87D

Archaeal biofilm regulator 1 (AbfR1) mutant Y84E S87D phosphomimic mutant
MWI(0) 32 kDa
MWexpected 26 kDa
VPorod 51 nm3
log I(s) 3.22×101 3.22×100 3.22×10-1 3.22×10-2
Archaeal biofilm regulator 1 (AbfR1) mutant Y84E S87D phosphomimic mutant small angle scattering data  s, nm-1
ln I(s)
Archaeal biofilm regulator 1 (AbfR1) mutant Y84E S87D phosphomimic mutant Guinier plot ln 3.22×101 Rg: 2.6 nm 0 (2.6 nm)-2 s2
(sRg)2I(s)/I(0)
Archaeal biofilm regulator 1 (AbfR1) mutant Y84E S87D phosphomimic mutant Kratky plot 1.104 0 √3 sRg
p(r)
Archaeal biofilm regulator 1 (AbfR1) mutant Y84E S87D phosphomimic mutant pair distance distribution function Rg: 2.7 nm 0 Dmax: 9.5 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Archaeal biofilm regulator 1 (AbfR1) mutant Y84E S87D phosphomimic mutant DAMMIN model
X-ray synchrotron radiation scattering data from solutions of N terminal affinity tag cleaved (by thrombin) AbfR1 Y84E S87D phosphomimic mutant in 20mMHEPES pH7.5, 200mM NaCl were collected on the BM29 beam line on the storage ring ESRF (Grenoble, France) using a 2D Photon counting Pilatus 1M pixel detector (s = 4π sin θ/λ, where 2θ is the scattering angle). A 3.8 mg/mL concentration was measured. 10 successive 20 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the different curves were scaled for protein concentration. The analysis was performed using the ATSAS package. The displayed model is an averaged and volume corrected dummy atom representation (DAMFILT) after imposing P2 symmetry.

Archaeal biofilm regulator 1 (AbfR1) mutant Y84E S87D phosphomimic mutant (ABfR1 mutant Y84E S8)
Mol. type   Protein
Organism   Sulfolobus acidocaldarius
Olig. state   Dimer
Mon. MW   13.2 kDa
 
UniProt   Q4JBH1 (1-116)
Sequence   FASTA