Synchrotron SAXS
data from solutions of
Cyclohexanone monooxygenase, NADP+ and ε-caprolactone, W492A
in
50 mM Tris 5 mM NADP+ 5 mM ε-caprolactone, pH 8
were collected
on the
12.3.1 (SIBYLS) beam line
at the Advanced Light Source (ALS) storage ring
(Berkeley, CA, USA)
using a MAR 165 CCD detector
at a sample-detector distance of 1.5 m and
at a wavelength of λ = 0.1 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
Solute concentrations ranging between 2.3 and 9 mg/ml were measured
at 20°C.
Four successive
frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.
Storage temperature = UNKNOWN. X-ray Exposure time = UNKNOWN
s, nm-1
