SASDBM9 – YezG: a cognate immunity protein of YeeF from Bacillus subtilis

YezG, cognate immunity protein of YeeF

MW^experimental	26	kDa
MW^expected	19	kDa
V^Porod	46	nm³

log I(s) 6.93×10⁴ 6.93×10³ 6.93×10² 6.93×10¹

YezG, cognate immunity protein of YeeF small angle scattering data

s, nm^-1

Log plot Log-Log plot

ln I(s)

YezG, cognate immunity protein of YeeF Guinier plot

ln 6.93×10⁴ R_g: 2.1 nm 0 (2.1 nm)^-2 s²

(sR_g)²I(s)/I(0)

YezG, cognate immunity protein of YeeF Kratky plot

1.104 0 √3 sR_g

p(r)	R_g: 2.1 nm 0 D_max: 6.1 nm

Data validation

Experimental data range

p(r) fit to data

Metric

Value

s_min D_max / π

0.3

N_Shannon

Worse

Better

Metric

Value

p_cormap

χ²

0.054
0.529

Worse

Better

Fits and models

log I(s)	s, nm^-1
+3 Δ/σ -3	s, nm^-1

YezG, cognate immunity protein of YeeF DAMMIN model

SAXS data from solutions of YezG in 20 mM HEPES 150 mM NaCl, pH 7.5 were collected on the IMTECH camera (Chandigarh, India) using a Diode 1D CMOS Mythen detector at a wavelength of λ = 0.15418 nm (I(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle). A single solute concentration of 10 mg/ml was measured at 25°C for 1800 seconds. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted to produce the final scattering profile for this entry.

Sample detector distance = UNKNOWN

YezG, cognate immunity protein of YeeF (YezG)
Mol. type		Protein
Organism		Bacillus subtilis subsp. spizizenii
Olig. state		Monomer
Mon. MW		19.2 kDa

UniProt		E0TU95
Sequence		FASTA