Structural Characterization of Cell Wall and Plasma Membrane Proteins of Arabidopsis thaliana

Haifa El Kilani, University of Hamburg Dissertation 8022 (2016) URL

SASDBN4 – Callose synthase

Callose synthase
MWexperimental 623 kDa
MWexpected 633 kDa
VPorod 1033 nm3
log I(s) 2.53×104 2.53×103 2.53×102 2.53×101
Callose synthase small angle scattering data  s, nm-1
ln I(s)
Callose synthase Guinier plot ln 2.54×104 Rg: 8.0 nm 0 (8.0 nm)-2 s2
(sRg)2I(s)/I(0)
Callose synthase Kratky plot 1.104 0 √3 sRg
p(r)
Callose synthase pair distance distribution function Rg: 7.9 nm 0 Dmax: 30 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Callose synthase CORAL model
log I(s)
 s, nm-1
Callose synthase DAMMIF model
Synchrotron SAXS data from solutions of Callose synthase in Tris, 50 mM NaCl, pH 7.3 were collected on the EMBL P12 beam line at the PETRA III storage ring (DESY; Hamburg, Germany) using a Pilatus 2M detector at a sample-detector distance of 3.1 m and at a wavelength of λ = 0.12 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 1.00 mg/ml was measured at 10°C. 18 successive 0.050 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Callose synthase (AtGSLO5-IL)
Mol. type   Protein
Organism   Arabidopsis thaliana
Olig. state   Octamer
Mon. MW   79.1 kDa
 
UniProt   Q9ZT82 (570-1208)
Sequence   FASTA