YeeF, a polymorphic toxin from Bacillus subtilis, is a ribosomal RNase with promiscuous DNA binding property

Krishan Gopal.

SASDBN9 – Putative DNA binding protein YeeF-CT in complex with the cognate immunity protein YezG from Bacillus subtilis

Putative DNA binding protein
YezG, cognate immunity protein of YeeF
MWexperimental 72 kDa
MWexpected 56 kDa
VPorod 115 nm3
log I(s) 7.27×104 7.27×103 7.27×102 7.27×101
Putative DNA binding protein YezG, cognate immunity protein of YeeF small angle scattering data  s, nm-1
ln I(s)
Putative DNA binding protein YezG, cognate immunity protein of YeeF Guinier plot ln 7.27×104 Rg: 4.0 nm 0 (4.0 nm)-2 s2
(sRg)2I(s)/I(0)
Putative DNA binding protein YezG, cognate immunity protein of YeeF Kratky plot 1.104 0 √3 sRg
p(r)
Putative DNA binding protein YezG, cognate immunity protein of YeeF pair distance distribution function Rg: 4.2 nm 0 Dmax: 14.1 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Putative DNA binding protein YezG, cognate immunity protein of YeeF DAMMIN model
SAXS data from solutions of Putative DNA binding protein YeeF-CT complexed with the cognate immunity protein YezG from Bacillus subtilis in 20 mM HEPES 150 mM NaCl, pH 7.5 were collected on the IMTECH camera (Chandigarh, India) using a Diode 1D CMOS Mythen detector at a wavelength of λ = 0.15418 nm (I(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle). A single solute concentration of 7.5 mg/ml was measured at 25°C for 1800 seconds. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted to produce the final scattering profile for this entry.

Sample detector distance = UNKNOWN

Putative DNA binding protein (YeeF)
Mol. type   Protein
Organism   Bacillus subtilis subsp. spizizenii
Olig. state   Dimer
Mon. MW   18.4 kDa
 
UniProt   E0TU96
Sequence   FASTA
 
YezG, cognate immunity protein of YeeF (YezG)
Mol. type   Protein
Organism   Bacillus subtilis subsp. spizizenii
Olig. state   Monomer
Mon. MW   19.2 kDa
 
UniProt   E0TU95
Sequence   FASTA