Structural basis of the interaction between the putative adhesion-involved and iron-regulated FrpD and FrpC proteins of Neisseria meningitidis.

Sviridova E, Rezacova P, Bondar A, Veverka V, Novak P, Schenk G, Svergun DI, Kuta Smatanova I, Bumba L, Sci Rep 7:40408 (2017) Europe PMC

SASDBP4 – Neisseria meningitidis iron-regulated outer membrane lipoprotein FrpD

Iron-regulated outer membrane lipoprotein FrpD
MWexperimental 13 kDa
MWexpected 27 kDa
VPorod 41 nm3
log I(s) 1.66×101 1.66×100 1.66×10-1 1.66×10-2
Iron-regulated outer membrane lipoprotein FrpD small angle scattering data  s, nm-1
ln I(s)
Iron-regulated outer membrane lipoprotein FrpD Guinier plot ln 1.66×101 Rg: 2.2 nm 0 (2.2 nm)-2 s2
(sRg)2I(s)/I(0)
Iron-regulated outer membrane lipoprotein FrpD Kratky plot 1.104 0 √3 sRg
p(r)
Iron-regulated outer membrane lipoprotein FrpD pair distance distribution function Rg: 2.1 nm 0 Dmax: 6.5 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Iron-regulated outer membrane lipoprotein FrpD DAMMIN model
log I(s)
 s, nm-1
Iron-regulated outer membrane lipoprotein FrpD PDB (PROTEIN DATA BANK) model
Synchrotron SAXS data from solutions of Neisseria meningitidis iron-regulated outer membrane lipoprotein FrpD in 10 mM Tris-HCl, 150 mM NaCl, 0.01% NaN3 were collected on the X33 beam line at the DORIS storage ring (DESY, Hamburg, Germany) using a Pilatus 1M-W detector (I(s) vs s; s = 4π sin θ/λ, where 2θ is the scattering angle and λ=0.15 nm). The data displayed in this entry were measured from one sample at a solute concentration of 8.4 mg/ml using an exposure time of 120 s (collected as 8 x 15 s frames). The data were normalized to the intensity of the transmitted beam and radially averaged and the scattering from the matched solvent-blank was subtracted. The data were normalized to protein concentration.

Cell temperature = UNKNOWN

Tags: X33
Iron-regulated outer membrane lipoprotein FrpD (FrpD)
Mol. type   Protein
Organism   Neisseria meningitidis
Olig. state   Monomer
Mon. MW   26.8 kDa
 
UniProt   Q08840 (43-271)
Sequence   FASTA
 
PDB ID   5EDJ