Disease Variants of FGFR3 Reveal Molecular Basis for the Recognition and Additional Roles for Cdc37 in Hsp90 Chaperone System.

Bunney TD, Inglis AJ, Sanfelice D, Farrell B, Kerr CJ, Thompson GS, Masson GR, Thiyagarajan N, Svergun DI, Williams RL, Breeze AL, Katan M, Structure 26(3):446-458.e8 (2018) Europe PMC

SASDBR9 – Human fibroblast growth factor receptor 3, from SEC-SAXS

Fibroblast growth factor receptor 3
MWexperimental 29 kDa
MWexpected 35 kDa
VPorod 59 nm3
log I(s) 1.83×102 1.83×101 1.83×100 1.83×10-1
Fibroblast growth factor receptor 3 small angle scattering data  s, nm-1
ln I(s)
Fibroblast growth factor receptor 3 Guinier plot ln 1.83×102 Rg: 2.4 nm 0 (2.4 nm)-2 s2
(sRg)2I(s)/I(0)
Fibroblast growth factor receptor 3 Kratky plot 1.104 0 √3 sRg
p(r)
Fibroblast growth factor receptor 3 pair distance distribution function Rg: 2.4 nm 0 Dmax: 8.3 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Fibroblast growth factor receptor 3 CORAL model
log I(s)
 s, nm-1
Fibroblast growth factor receptor 3 MONSA model
Synchrotron SAXS data from solutions of Human fibroblast growth factor receptor 3 in 25 mM Tris-HCl, 150 mM NaCl, 5% (v/v) glycerol, 1 mM TCEP, pH 8, were collected using size-exclusion chromatography SAXS (SEC-SAXS) on the P12 beam line at the PETRA III storage ring (Hamburg, Germany) equipped with a Pilatus 2M detector at a sample-detector distance of 3.1 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle). The scattering pattern was extracted from the SEC-SAXS intensity data (weighted for the estimated errors) using the non-negative matrix factorization (NMF) algorithm as implemented in the scikit-learn package. The NMF fit was done with four components (one of which corresponds to the buffer), and the component with Guinier radius closest to that expected for FGFR3 was selected for further analysis.

Concentration min = UNKNOWN

Fibroblast growth factor receptor 3 (FGFR3)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   35.5 kDa
 
UniProt   P22607 (455-768)
Sequence   FASTA
 
PDB ID   4K33