Hybrid Structural Analysis of the Arp2/3 Regulator Arpin Identifies Its Acidic Tail as a Primary Binding Epitope.

Fetics S, Thureau A, Campanacci V, Aumont-Nicaise M, Dang I, Gautreau A, Pérez J, Cherfils J, Structure 24(2):252-60 (2016) Europe PMC

SASDBW2 – Zebrafish (Danio rerio) Arpin truncated C-terminal mutant (delta-C 16).

Zebrafish (Danio rerio) Arpin truncated C-terminal mutant (delta-C 16).
MWexperimental 26 kDa
MWexpected 24 kDa
VPorod 37 nm3
log I(s) 6.60×10-2 6.60×10-3 6.60×10-4 6.60×10-5
Zebrafish (Danio rerio) Arpin truncated C-terminal mutant (delta-C 16). small angle scattering data  s, nm-1
ln I(s)
Zebrafish (Danio rerio) Arpin truncated C-terminal mutant (delta-C 16). Guinier plot ln 6.60×10-2 Rg: 2.2 nm 0 (2.2 nm)-2 s2
(sRg)2I(s)/I(0)
Zebrafish (Danio rerio) Arpin truncated C-terminal mutant (delta-C 16). Kratky plot 1.104 0 √3 sRg
p(r)
Zebrafish (Danio rerio) Arpin truncated C-terminal mutant (delta-C 16). pair distance distribution function 0 Dmax: 11.3 nm

Data validation

There are no models related to this curve.

X-ray synchrotron radiation scattering data from solutions of zebrafish arpin (truncated C-terminal mutant lacking the last C-terminal 16 amino acids of the wild-type sequence) in 50mM HEPES, pH 7.5, 100mM NaCl, 1mM TCEP were collected at 15 C on the SWING Beamline, Synchrotron Soleil, Gif Sur Yvette, France using a PCCD170170 Aviex detector. These data are derived from size-exclusion chromatography-SAXS. Note: The original SAXS data where the momentum transfer s in inverse Angstroms (s = 4π sin θ/λ, where 2θ is the scattering angle) is included in the zip file associated with this entry.

Due to high concentration at the elution peak, the HPLC UV detector was saturated and the concentrations of the sample was consequently underestimated. In this case, the molecular mass is overestimated when assessing this parameter from I(0) and concentration.

Zebrafish (Danio rerio) Arpin truncated C-terminal mutant (delta-C 16). (Arpin-deltaC)
Mol. type   Protein
Organism   Danio rerio
Olig. state   Monomer
Mon. MW   23.8 kDa
 
UniProt   Q1LWJ6 (1-210)
Sequence   FASTA