Allosteric communication between DNA-binding and light-responsive domains of diatom class I aureochromes.

Banerjee A, Herman E, Serif M, Maestre-Reyna M, Hepp S, Pokorny R, Kroth PG, Essen LO, Kottke T, Nucleic Acids Res 44(12):5957-70 (2016) Europe PMC

SASDBX3 – Aureobox dsDNA

Aureobox dsDNA
MWI(0) 9 kDa
MWexpected 13 kDa
VPorod 18 nm3
log I(s) 2.14×101 2.14×100 2.14×10-1 2.14×10-2
Aureobox dsDNA small angle scattering data  s, nm-1
ln I(s)
Aureobox dsDNA Guinier plot ln 2.15×101 Rg: 2.0 nm 0 (2.0 nm)-2 s2
(sRg)2I(s)/I(0)
Aureobox dsDNA Kratky plot 1.104 0 √3 sRg
p(r)
Aureobox dsDNA pair distance distribution function Rg: 2.2 nm 0 Dmax: 8.6 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Aureobox dsDNA DAMFILT model
X-ray synchrotron radiation scattering data from solution of Aureo box dsDNA in 0.5X TBE (50 mM Tris pH 8, 50 mM boric acid, 1 mM EDTA) buffer was collected on the BM29 camera on the storage ring ESRF (Grenoble, France) using a 2D Photon counting Pilatus 1M-W pixel detector (s = 4π sin θ/λ, where 2θ is the scattering angle). Ten successive 20 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. Solution scattering data of Aureo box dsDNA was performed at 1.25mg/mL concentration at 4 degrees centigrade. The modelling analysis was performed using the ATSAS package. The displayed model is an averaged and volume corrected dummy atom representation (DAMFILT).

Aureobox dsDNA
Mol. type   DNA
Organism   synthetic construct
Olig. state   Monomer
Mon. MW   13.0 kDa
Sequence   FASTA