Apple Snail Perivitellin Precursor Properties Help Explain Predators' Feeding Behavior.

Cadierno MP, Dreon MS, Heras H, Physiol Biochem Zool 90(4):461-470 (2017) Europe PMC

SASDBX4 – Albumen gland precursor of perivitellin-2 (PcPV2)

Perivitellin-2 31 kDa subunit
Perivitellin-2 67 kDa subunit
MWexperimental 400 kDa
MWexpected 376 kDa
VPorod 294 nm3
log I(s) 4.90×10-4 4.90×10-5 4.90×10-6 4.90×10-7
Perivitellin-2 31 kDa subunit Perivitellin-2 67 kDa subunit small angle scattering data  s, nm-1
ln I(s)
Perivitellin-2 31 kDa subunit Perivitellin-2 67 kDa subunit Guinier plot ln 4.90×10-4 Rg: 4.8 nm 0 (4.8 nm)-2 s2
(sRg)2I(s)/I(0)
Perivitellin-2 31 kDa subunit Perivitellin-2 67 kDa subunit Kratky plot 1.104 0 √3 sRg
p(r)
Perivitellin-2 31 kDa subunit Perivitellin-2 67 kDa subunit pair distance distribution function Rg: 4.9 nm 0 Dmax: 17.0 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Perivitellin-2 31 kDa subunit Perivitellin-2 67 kDa subunit DAMMIF model
log I(s)
 s, nm-1
Perivitellin-2 31 kDa subunit Perivitellin-2 67 kDa subunit DAMMIF model
X-ray synchrotron radiation scattering data from solutions of the albumen gland precursor of perivitellin-2 (PcPV2) in 20 mM Tris-HCl, pH 8.5 on the D02A-SAXS2 Beamline at the Laboratorio Nacional de Luz Sincrotron (LNLS, Campinas, Brazil) using a MAR Image Plate detector (I(s) vs s, where s = 4π sin θ/λ, 2θ is the scattering angle and λ the X-ray wavelength, 0.155 nm). Different solute concentrations in the range 0.60-2.40 mg/ml were measured. Five successive 300 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the different curves were scaled for protein concentration. The low angle data collected were obtained from a single concentration scattering curve. PcPV2 is an oligomeric protein of 400kDa composed of the perivitellin-2 31 kDa and perivitellin-2 67 kDa subunits.

Wavelength = UNKNOWN. Sample detector distance = UNKNOWN

Perivitellin-2 31 kDa subunit (PcPV2-31)
Mol. type   Protein
Organism   Pomacea canaliculata
Olig. state   Tetramer
Mon. MW   31.6 kDa
 
UniProt   P0C8G7
Sequence   FASTA
 
Perivitellin-2 67 kDa subunit (PcPV2-67)
Mol. type   Protein
Organism   Pomacea canaliculata
Olig. state   Tetramer
Mon. MW   62.5 kDa
 
UniProt   P0C8G6
Sequence   FASTA