Structural model of the cytosolic domain of the plant ethylene receptor 1 (ETR1).

Mayerhofer H, Panneerselvam S, Kaljunen H, Tuukkanen A, Mertens HD, Mueller-Dieckmann J, J Biol Chem 290(5):2644-58 (2015) Europe PMC

SASDC32 – Ethylene Receptor 1 Cytosolic Domain (in 250 mM NDSB)

Ethylene Receptor 1
MWI(0) 54 kDa
MWexpected 129 kDa
VPorod 316 nm3
log I(s) 6.78×101 6.78×100 6.78×10-1 6.78×10-2
Ethylene Receptor 1 small angle scattering data  s, nm-1
ln I(s)
Ethylene Receptor 1 Guinier plot ln 6.78×101 Rg: 4.7 nm 0 (4.7 nm)-2 s2
(sRg)2I(s)/I(0)
Ethylene Receptor 1 Kratky plot 1.104 0 √3 sRg
p(r)
Ethylene Receptor 1 pair distance distribution function Rg: 5.2 nm 0 Dmax: 15.8 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Ethylene Receptor 1 Cytosolic Domain (in 250 mM NDSB) Rg histogram Rg, nm
log I(s)
 s, nm-1
Ethylene Receptor 1 CORAL model
log I(s)
 s, nm-1
log I(s)
 s, nm-1
log I(s)
 s, nm-1
Ethylene Receptor 1 MONSA model
log I(s)
 s, nm-1
log I(s)
 s, nm-1
Synchrotron SAXS data from solutions of Ethylene Receptor 1 Cytosolic Domain (in 250 mM NDSB) in 20 mM Tris-NDSB 150 mM NaCl 1mM DTT 250 mM NDSB, pH 8.8 were collected on the EMBL X33 beam line at the DORIS III, DESY storage ring (Hamburg, Germany) using a Pilatus 1M-W detector at a sample-detector distance of 3.1 m and at a wavelength of λ = 0.15 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 0.7 and 2.7 mg/ml were measured at 10°C. Eight successive 15 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentrations were extrapolated to infinite dilution and merged with the higher concentration data to yield the final composite scattering curve.

Cytosolic domain of ETR1 in 250 mM NBSB

Tags: X33
Ethylene Receptor 1 (ETR1(-ΔTM))
Mol. type   Protein
Organism   Arabidopsis thaliana
Olig. state   Dimer
Mon. MW   64.6 kDa
 
UniProt   P49333 (158-738)
Sequence   FASTA