| MWI(0) | 53 | kDa |
| MWexpected | 129 | kDa |
| VPorod | 274 | nm3 |
|
log I(s)
1.12×103
1.12×102
1.12×101
1.12×100
|
s, nm-1
|
Structural model of the cytosolic domain of the plant ethylene receptor 1 (ETR1).
Mayerhofer H, Panneerselvam S, Kaljunen H, Tuukkanen A, Mertens HD, Mueller-Dieckmann J, J Biol Chem 290(5):2644-58 (2015) Europe PMCSASDC42 – Ethylene Receptor 1 Cytosolic Domain
Ethylene Receptor 1|
|
|
Fits and models
|
Rg, nm
|
|
Synchrotron SAXS
data from solutions of
Ethylene Receptor 1 Cytosolic Domain
in
20 mM TRIS 150 mM NaCl 1mM DTT 250mM NDSB, pH 8.8
were collected
on the
EMBL P12 beam line
at the PETRA III storage ring
(DESY; Hamburg, Germany)
using a Pilatus 2M detector
at a sample-detector distance of 3.1 m and
at a wavelength of λ = 1.24 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
Solute concentrations ranging between 0.7 and 4.2 mg/ml were measured
at 10°C.
20 successive
0.050 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.
ETR1 in the absence of NDSB |
|
|||||||||||||||||||||||||||