Structural and functional dissection of the DH and PH domains of oncogenic Bcr-Abl tyrosine kinase.

Reckel S, Gehin C, Tardivon D, Georgeon S, Kükenshöner T, Löhr F, Koide A, Buchner L, Panjkovich A, Reynaud A, Pinho S, Gerig B, Svergun D, Pojer F, Güntert P, Dötsch V, Koide S, Gavin AC, Hantschel O, Nat Commun 8(1):2101 (2017) Europe PMC

SASDC46 – PH - Pleckstrin-homology domain of Bcr-Abl tyrosine kinase p210

BCR-ABL p210 fusion protein (PH domain)
MWI(0) 16 kDa
MWexpected 22 kDa
VPorod 38 nm3
log I(s) 2.07×103 2.07×102 2.07×101 2.07×100
BCR-ABL p210 fusion protein (PH domain) small angle scattering data  s, nm-1
ln I(s)
BCR-ABL p210 fusion protein (PH domain) Guinier plot ln 2.07×103 Rg: 2.0 nm 0 (2.0 nm)-2 s2
(sRg)2I(s)/I(0)
BCR-ABL p210 fusion protein (PH domain) Kratky plot 1.104 0 3 sRg
p(r)
BCR-ABL p210 fusion protein (PH domain) pair distance distribution function Rg: 2.1 nm 0 Dmax: 6.6 nm

Data validation


Fits and models


log I(s)
 s, nm-1
BCR-ABL p210 fusion protein (PH domain) SREFLEX model

Synchrotron SAXS data from solutions of PH - Pleckstrin-homology domain of Bcr-Abl tyrosine kinase p210 in 25 mM Tris-HCl, 150 mM NaCl, 5% Glycerol, 1 mM DTT, pH 7.5 were collected on the EMBL P12 beam line at the PETRA III storage ring (DESY; Hamburg, Germany) using a Pilatus 2M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 1 and 9.1 mg/ml were measured at 10°C. 20 successive 0.045 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.

The homology model is derived from PDB ID: 2z0q. The PDB entry for BCR-ABL p210 fusion protein PH domain is 5oc7 (has an internal deletion).

BCR-ABL p210 fusion protein (PH domain)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   22.4 kDa
 
UniProt   P11274
Sequence   FASTA