| MWI(0) | 35 | kDa |
| MWexpected | 54 | kDa |
| VPorod | 117 | nm3 |
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log I(s)
2.50×10-2
2.50×10-3
2.50×10-4
2.50×10-5
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s, nm-1
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Structural model of human dUTPase in complex with a novel proteinaceous inhibitor.
Nyíri K, Mertens HDT, Tihanyi B, Nagy GN, Kőhegyi B, Matejka J, Harris MJ, Szabó JE, Papp-Kádár V, Németh-Pongrácz V, Ozohanics O, Vékey K, Svergun DI, Borysik AJ, Vértessy BG, Sci Rep 8(1):4326 (2018) Europe PMCSASDC57 – Deoxyuridine 5'-triphosphate nucleotidohydrolase
Deoxyuridine 5'-triphosphate nucleotidohydrolase|
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Fits and models
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Synchrotron SAXS
data from solutions of
Deoxyuridine 5'-triphosphate nucleotidohydrolase
in
50 mM HEPES 300 mM NaCl 5 mM MgCl2, pH 7.5
were collected
on the
EMBL P12 beam line
at the PETRA III storage ring
(DESY; Hamburg, Germany)
using a Pilatus 2M detector
at a sample-detector distance of 3.1 m and
at a wavelength of λ = 0.124 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
Solute concentrations ranging between 0.2 and 2 mg/ml were measured
at 10°C.
20 successive
0.050 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.
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