| MWexperimental | 46 | kDa |
| MWexpected | 58 | kDa |
| VPorod | 74 | nm3 |
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log I(s)
6.97×101
6.97×100
6.97×10-1
6.97×10-2
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s, nm-1
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The switch that does not flip: the blue-light receptor YtvA from Bacillus subtilis adopts an elongated dimer conformation independent of the activation state as revealed by a combined AUC and SAXS study.
Jurk M, Dorn M, Kikhney A, Svergun D, Gärtner W, Schmieder P, J Mol Biol 403(1):78-87 (2010) Europe PMCSASDC66 – Blue light receptor YtvA, lit/active state
pfyP - Blue light photoreceptor|
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Fits and models
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Synchrotron SAXS
data from solutions of
Blue light receptor YtvA, lit/active state
in
PBS + 5 mM DTT, pH 7.4
were collected
on the
EMBL X33 beam line
at the DORIS III, DESY storage ring
(Hamburg, Germany)
using a Pilatus 1M-W detector
at a sample-detector distance of 2.7 m and
at a wavelength of λ = 0.15 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
Solute concentrations ranging between 1.7 and 11 mg/ml were measured
at 20°C.
Four successive
30 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
The low angle data collected at lower concentrations were extrapolated to infinite dilution and merged with the higher concentration data to yield the final composite scattering curve.
Tags:
X33
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