Molecular Organization of Soluble Type III Secretion System Sorting Platform Complexes.

Bernal I, Börnicke J, Heidemann J, Svergun D, Horstmann JA, Erhardt M, Tuukkanen A, Uetrecht C, Kolbe M, J Mol Biol 431(19):3787-3803 (2019) Europe PMC

SASDC68 – Surface presentation of antigens protein, SpaOc

Surface presentation of antigens protein SpaO SpaO(SPOA2)
MWexperimental 23 kDa
MWexpected 25 kDa
VPorod 51 nm3
log I(s) 4.56×103 4.56×102 4.56×101 4.56×100
Surface presentation of antigens protein SpaO SpaO(SPOA2) small angle scattering data  s, nm-1
ln I(s)
Surface presentation of antigens protein SpaO SpaO(SPOA2) Guinier plot ln 4.56×103 Rg: 2.5 nm 0 (2.5 nm)-2 s2
(sRg)2I(s)/I(0)
Surface presentation of antigens protein SpaO SpaO(SPOA2) Kratky plot 1.104 0 √3 sRg
p(r)
Surface presentation of antigens protein SpaO SpaO(SPOA2) pair distance distribution function Rg: 2.7 nm 0 Dmax: 9.1 nm

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of Surface presentation of antigens protein, SpaOc in 20 mM HEPES pH 7.5, 150 mM NaCl, pH 7.5 were collected on the EMBL P12 beam line at the PETRA III storage ring (DESY; Hamburg, Germany) using a Pilatus 2M detector at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 14.80 mg/ml was measured at 20°C. 3600 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Sample detector distance = UNKNOWN

Surface presentation of antigens protein SpaO SpaO(SPOA2) (SpaOc)
Mol. type   Protein
Organism   Salmonella enterica subsp. enterica serovar Typhimurium
Olig. state   Dimer
Mon. MW   12.4 kDa
 
UniProt   P40699 (203-303)
Sequence   FASTA