Rizzo AA,
Vassel FM,
Chatterjee N,
D'Souza S,
Li Y,
Hao B,
Hemann MT,
Walker GC,
Korzhnev DM,
Proc Natl Acad Sci U S A
115(35):E8191-E8200
(2018)
Europe PMC
SASDC69 – Human Rev7 dimer in complex with Rev3 peptide @ 4.6mg/mL
Dmax unknown – experimental data range validation not possible.
There are no models related to this curve.
Synchrotron SAXS
data from solutions of
Human Rev7 dimer in complex with Rev3 peptide @ 4.6mg/mL
in
20 mM HEPES, 10 mM DTT, 5% glycerol, pH 8
were collected
on the
G1 beam line
at the Cornell High Energy Synchrotron Source (CHESS) storage ring
(Ithaca, NY, USA)
using a Finger Lakes CCD detector
at a wavelength of λ = 0.1267 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
One solute concentration of 4.60 mg/ml was measured
at 4°C.
10 successive
1 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
Based on the affinity of the Rev7 dimer, at this concentration (and below) there is a mixture of monomer and dimer in the solution. This dataset was not used for modeling
s, nm-1
