| MWexperimental | 90 | kDa |
| MWexpected | 90 | kDa |
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log I(s)
3.46×10-1
3.46×10-2
3.46×10-3
3.46×10-4
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s, nm-1
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Molecular basis of human CD22 function and therapeutic targeting.
Ereño-Orbea J, Sicard T, Cui H, Mazhab-Jafari MT, Benlekbir S, Guarné A, Rubinstein JL, Julien JP, Nat Commun 8(1):764 (2017) Europe PMCSASDC76 – Extracellular domain of human B-lymphocyte cell receptor CD22
CD22 extracellular domain|
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Fits and models
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Synchrotron SAXS
data from solutions of
Extracellular domain of human B-lymphocyte cell receptor CD22
in
20 mM Tris 150 mM NaCl, pH 9
were collected
on the
12-ID-B SAXS/WAXS beam line
at the Advanced Photon Source (APS), Argonne National Laboratory storage ring
(Lemont, IL, USA)
using a Pilatus 2M detector
at a sample-detector distance of 3.6 m and
at a wavelength of λ = 0.0886 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
One solute concentration of 5.00 mg/ml was measured
at 22°C.
30 successive
1 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
Storage temperature = UNKNOWN |
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