Molecular Organization of Soluble Type III Secretion System Sorting Platform Complexes.

Bernal I, Börnicke J, Heidemann J, Svergun D, Horstmann JA, Erhardt M, Tuukkanen A, Uetrecht C, Kolbe M, J Mol Biol 431(19):3787-3803 (2019) Europe PMC

SASDC78 – Surface presentation of antigens protein SpaOc-SpaO(SPOA1,2)

Surface presentation of antigens protein SpaO SpaO(SPOA2)
Surface presentation of antigens protein SpaO(SPOA1,2)
MWexperimental 56 kDa
MWexpected 59 kDa
VPorod 108 nm3
log I(s) 4.37×103 4.37×102 4.37×101 4.37×100
Surface presentation of antigens protein SpaO SpaO(SPOA2) Surface presentation of antigens protein SpaO(SPOA1,2) small angle scattering data  s, nm-1
ln I(s)
Surface presentation of antigens protein SpaO SpaO(SPOA2) Surface presentation of antigens protein SpaO(SPOA1,2) Guinier plot ln 4.37×103 Rg: 3.3 nm 0 (3.3 nm)-2 s2
(sRg)2I(s)/I(0)
Surface presentation of antigens protein SpaO SpaO(SPOA2) Surface presentation of antigens protein SpaO(SPOA1,2) Kratky plot 1.104 0 √3 sRg
p(r)
Surface presentation of antigens protein SpaO SpaO(SPOA2) Surface presentation of antigens protein SpaO(SPOA1,2) pair distance distribution function Rg: 3.3 nm 0 Dmax: 11.1 nm

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of Surface presentation of antigens protein SpaOc-SpaO(SPOA1,2) in 20 mM HEPES pH 7.5, 150 mM NaCl, pH 7.5 were collected on the EMBL P12 beam line at the PETRA III storage ring (DESY; Hamburg, Germany) using a Pilatus 2M detector at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 7.40 mg/ml was measured at 20°C. 3600 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Sample detector distance = UNKNOWN

Surface presentation of antigens protein SpaO SpaO(SPOA2) (SpaOc)
Mol. type   Protein
Organism   Salmonella enterica subsp. enterica serovar Typhimurium
Olig. state   Dimer
Mon. MW   12.4 kDa
 
UniProt   P40699 (203-303)
Sequence   FASTA
 
Surface presentation of antigens protein SpaO(SPOA1,2) (SpaO)
Mol. type   Protein
Organism   Salmonella enterica subsp. enterica serovar Typhimurium
Olig. state   Monomer
Mon. MW   33.8 kDa
 
UniProt   P40699 (1-303)
Sequence   FASTA